苏文凤;韦中亚;顾芸;沈筠恬;陈罡;

• 1:南通大学江苏省神经再生重点实验室

摘要(Abstract)：

目的:探讨施万细胞与背根节神经元髓鞘化共培养的标准化方法,为研究周围神经髓鞘化的形成机制提供稳定的周围神经髓鞘化体外模型。方法:取出生1³ d新生SD大鼠,培养施万细胞,经纯化鉴定后用于共培养。取孕143 d新生SD大鼠,培养施万细胞,经纯化鉴定后用于共培养。取孕14¹5 d的SD大鼠胚鼠背根神经节,经纯化后用于共培养;将2种分别纯化的细胞进行共培养,加抗坏血酸诱导髓鞘的形成。利用免疫组化染色、扫描电镜和透射电镜,检测髓鞘的形成。结果:纯化后施万细胞纯度可达到98%以上,可用于共培养。背根节神经元贴壁良好,经纯化后几乎无杂细胞可见,可用于共培养。对共培养细胞进行MAG与NF的免疫组化染色,发现有数量可观的髓鞘形成,并且髓鞘是紧密包绕在神经元轴突上。扫描电镜下可观察到施万细胞包绕轴突形成髓鞘,而透射电镜下则可观察到有致密的髓鞘板层结构形成。结论:本实验成功建立稳定可靠的体外成髓鞘模型,可用于轴突的新髓鞘化实验研究。

关键词(KeyWords)： 施万细胞;背根节神经元;髓鞘形成;免疫荧光染色法;大鼠

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(30800315,31071251);; 南通大学校级自然科学类科研基金项目(13Z008)

作者(Authors): 苏文凤;韦中亚;顾芸;沈筠恬;陈罡;

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