烧伤患者血清对骨髓间充质干细胞生物学特性的影响Effects of serum from normal and burned patients on biological behavior of bone marrow mesenchymal stem cells
胡克苏;张逸;顾志峰;姜津霞;杨惠林;
摘要(Abstract)：
目的:探讨烧伤患者血清对体外培养的骨髓间充质干细胞的生物学行为的影响。方法:培养BM-MSCs,传代后分别用含有体积分数为10%正常人血清、烧伤患者血清的低糖达氏修正依氏培养基培养,用噻唑蓝(MTT)法测定其生长曲线,碘化丙啶(PI)染色,流式细胞仪分析细胞周期、β-gal染色测定衰老的MSCs。结果:含正常血清培养基传代培养的细胞生长曲线较烧伤患者血清慢,烧伤患者血清处理的细胞中G0/G1期细胞比例明显低于正常血清组(P<0.01),β-gal染色阳性细胞数目显著低于正常血清组。结论:烧伤患者血清比正常血清能更好地促进BM-MSCs生长;烧伤血清内可能含有多种能影响BM-MSCs生长的物质。
关键词(KeyWords)： 骨髓间充质干细胞;烧伤;血清;细胞生物学
基金项目(Foundation): 江苏省六大人才高峰(第六批)资助课题;; 江苏省教育厅自然科学基金(09KJB320010);; 南通市社会发展科技计划(S2008028)
作者(Authors): 胡克苏;张逸;顾志峰;姜津霞;杨惠林;
参考文献(References)：
- [1]Pittenger MF,Mackay AM,Beck SC,et al.Multilineage po-tential of adult human mesenchymal stem cells[J].Science,1999,284(5411):143-147.
- [2]顾志峰,赵盛楠,张华勇,等.系统性红斑狼疮患者骨髓间质干细胞超微结构及细胞骨架的特征[J].中华风湿病学杂志,2008,12(6):397-400.
- [3]Tamama K,Fan VH,Griffith LG,et al.Epidermal growthfactor as a candidate for ex vivo expansion of bone marrow-derived mesenchymal stem cells[J].Stem Cells,2006,24(3):686-695.
- [4]Hashimoto J,Kariya Y,Miyazaki K,et al.Regulation of pro-liferation and chondrogenic differentiation of human mes-enchymal stem cells by laminin-5(laminin-332)[J].StemCells,2006,24(11):2346-2354.
- [5]Gruber R,Karreth F,Kandler B,et al.Platelet-released su-pernatants increase migration and proliferation,and decreaseosteogenic differentiation of bone marrow-derived mesenchy-mal progenitor cells under in vitro conditions[J].Platelet,2004,15(1):29-35.
- [6]韩冰,付小兵,梁雪梅,等.烧伤大鼠血清诱导骨髓间充质干细胞分化为表皮细胞和血管内皮细胞的实验研究[J].解放军医学杂志,2004,29(8):652-654.
- [7]韩冰,付小兵,陈伟,等.烧伤前后大鼠血清对骨髓基质细胞生物学行为的影响[J].中国危重急救医学,2003,15(10):603-605.