叶亚峻;朱小存;朱治淮;刘琪;秦凯;

• 1:淮安市肿瘤医院普通外科

摘要(Abstract)：

目的:研究蛋白质酪氨酸磷酸酶1B(PTP1B)在胃癌组织中的表达及其对胃癌细胞株MKN28增殖、侵袭的影响,为PTP1B作为靶点治疗胃癌提供理论基础。方法:采用Western blot检测PTP1B在39例胃癌组织及8例非癌胃组织中的表达情况,分析其与胃癌重要病理因素之间的关系,并通过构建靶向PTP1B的ShRNA的真核表达质粒Sh-PTP1B转染人胃癌细胞系MKN28,应用Western blot检测PTP1B蛋白表达,采用CCK-8、Transwell侵袭小室检测MKN28细胞增殖和侵袭。结果:PTP1B在胃癌组织的表达明显高于非癌胃组织,PTP1B表达与胃癌患者组织分化程度及TNM分期明显相关;胃癌细胞系MKN28中转染Sh-PTP1B后,细胞PTP1B蛋白表达明显减少,细胞增殖和侵袭明显抑制(P<0.05)。结论:PTP1B在胃癌的生长和侵袭过程中发挥重要作用,有可能成为靶向治疗胃癌的潜在靶点。

关键词(KeyWords)： 胃癌;RNA干扰;蛋白质酪氨酸磷酸酶1B

Abstract:

Keywords:

基金项目(Foundation):

作者(Authors): 叶亚峻;朱小存;朱治淮;刘琪;秦凯;

参考文献(References)：

• [1]王婕敏,林三仁.胃癌研究及诊治新进展[J].胃肠病学和肝病学杂志,2012,21(1):3-5.
• [2]Motiwala T,Jacob ST.Role of protein tyrosine phosphatases in cancer[J].Prog Nucleic Acid Res Mol Biol,2006,81:297-329.
• [3]Tonks NK,Diltz CD,Fischer EH.Purification of the major protein-tyrosine-phosphatases of human placenta[J].J Biol Chem,1988,263(14):6722-6730.
• [4]Buffart TE,van Grieken NC,Tijssen M,et al.High resolution analysis of DNA copy-number aberrations of chromosomes 8,13,and 20 in gastric cancers[J].Virchows Arch,2009,455(3):213-223.
• [5]Warabi M,Nemoto T,Ohashi K,et al.Expression of protein tyrosine phosphatases and its significance in esophageal Cancer[J].Exp Mol Pathol,2000,68(3):187-195.
• [6]黄卓,石琨.蛋白质酪氨酸磷酸酶1B及血管内皮素在卵巢癌中的表达[D].黑龙江哈尔滨:哈尔滨医科大学,2012.
• [7]Zhu S,Bjorge JD,Fujita DJ.PTP1B contributes to the oncogenic properties of colon cancer cells through Src activation[J].Cancer Res,2007,67(21):10129-10137.
• [8]Wang J,Liu B,Chen X,et al.PTP1B expression contributes to gastric cancer progression[J].Med Oncol,2012,29(2):948-956.
• [9]Butz K,Ristriani T,Hengstermann A,et al.siRNA targeting of the viral E6 oncogene efficiently kills human papillomavirus-positive cancer cells[J].Oncogene,2003,22(38):5938-5945.
• [10]Chen Y,Stamatoyannopoulos G,Song CZ.Down-regulation of CXCR4 by inducible small interfering RNA inhibits breast cancer cell invasion in vitro[J].Cancer Res,2003,63(16):4801-4804.
• [11]Grzmil M,Thelen P,Hemmerlein B,et al.Bax inhibitor-1is overexpressed in prostate cancer and its specific downregulation by RNA interference leads to cell death in human prostate carcinoma cells[J].Am J Pathol,2003,163(2):543-552.
• [12]Wang J,Chen X,Liu B,et al.Suppression of PTP1B in gastric cancer cells in vitro induces a change in the genome-wide expression profile and inhibits gastric cancer cell growth[J].Cell Biol Int,2010,34(7):747-753.