PTP1B在胃癌组织中表达及其对胃癌细胞系MKN28增殖、侵袭行为的影响Expression of PTP1B in gastric cancer and its effect on proliferation and invasion of gastric cancer cell line MKN28
叶亚峻;朱小存;朱治淮;刘琪;秦凯;
摘要(Abstract):
目的:研究蛋白质酪氨酸磷酸酶1B(PTP1B)在胃癌组织中的表达及其对胃癌细胞株MKN28增殖、侵袭的影响,为PTP1B作为靶点治疗胃癌提供理论基础。方法:采用Western blot检测PTP1B在39例胃癌组织及8例非癌胃组织中的表达情况,分析其与胃癌重要病理因素之间的关系,并通过构建靶向PTP1B的ShRNA的真核表达质粒Sh-PTP1B转染人胃癌细胞系MKN28,应用Western blot检测PTP1B蛋白表达,采用CCK-8、Transwell侵袭小室检测MKN28细胞增殖和侵袭。结果:PTP1B在胃癌组织的表达明显高于非癌胃组织,PTP1B表达与胃癌患者组织分化程度及TNM分期明显相关;胃癌细胞系MKN28中转染Sh-PTP1B后,细胞PTP1B蛋白表达明显减少,细胞增殖和侵袭明显抑制(P<0.05)。结论:PTP1B在胃癌的生长和侵袭过程中发挥重要作用,有可能成为靶向治疗胃癌的潜在靶点。
关键词(KeyWords): 胃癌;RNA干扰;蛋白质酪氨酸磷酸酶1B
基金项目(Foundation):
作者(Authors): 叶亚峻;朱小存;朱治淮;刘琪;秦凯;
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