孙驰;陶涛;

• 1:南通大学医学院

摘要(Abstract)：

目的:研究肿瘤坏死因子受体相关因子6(TRAF6)在小胶质细胞炎性激活中的作用。方法:分离培养大鼠原代小胶质细胞,利用脂多糖(LPS)处理构建炎症模型,免疫印迹检测TRAF6的表达改变;合成TRAF6的siRNA,转染小胶质细胞后构建LPS诱导的细胞炎性激活模型,检测细胞活化标记F4/80的表达。结果:脂多糖可诱导小胶质细胞中F4/80的表达,并存在浓度依赖性。干预细胞中TRAF6的表达抑制小胶质细胞中活化标记F4/80的表达。结论:TRAF6在小胶质细胞炎性活化过程中表达增加,干预TRAF6的表达抑制细胞炎性活化。

关键词(KeyWords)： 肿瘤坏死因子受体相关因子;小胶质细胞;脂多糖;Western Blot检测;大鼠

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(81202368)

作者(Authors): 孙驰;陶涛;

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