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目的：研究胃癌细胞中细胞骨架结构相关基因的表达及其在胃癌发生发展中的作用。方法：采用实时定量PCR(qRT-PCR)技术测定正常人胃黏膜上皮细胞系与胃癌细胞系中PLEC、FAT1、EZR、HMGA1、MISP和PLAUR基因表达水平。采用基因干扰技术沉默MKN-1细胞、SGC-7901细胞中PLEC、PLAUR基因表达，CCK8法测定siPLEC、si-PLAUR转染后细胞增殖能力。将si-NC和si-PLEC转染MKN-1细胞悬浮液植入裸鼠皮下，20天后观察裸鼠皮下肿瘤体积，并进行肿瘤组织HE染色。结果：qRT-PCR结果显示，与正常人胃上皮细胞比较，胃癌细胞系中PLEC、FAT1、EZR、HMGA1、MISP和PLAUR基因表达均显著上调（均P<0.05）。CCK8实验结果显示，si-PLEC、siPLAUR组细胞增殖能力显著低于si-NC组（均P<0.05）。干扰PLEC表达的胃癌细胞形成的瘤体体积明显小于对照组，与si-PLEC裸鼠比较，si-NC裸鼠肿瘤组织呈现更明显的异型性。结论：PLEC、FAT1、EZR、HMGA1、MISP和PLAUR等蛋白在胃癌发生发展中具有重要作用，可能作为潜在的分子标志物用于胃癌早期诊断及靶向治疗。
Abstract：Objective:To investigate the expression characteristics of molecular biomarkers in gastric cancer cells and their roles in the occurrence and progression of gastric cancer. Methods: The expression levels of genes such as PLEC, FAT1, EZR, HMGA1, MISP and PLAUR in normal human gastric epithelial cells and gastric cancer cells were determined using real-time quantitative PCR(qRT-PCR) technology. The expressions of PLEC and PLAUR genes in MKN-1 and SGC-7901 cells were respectively silenced by gene interference technology, and the proliferation abilities of cells in the si-PLEC, si-PLAUR and si-NC groups were determined by CCK8 method. Results: qRT-PCR results showed that compared with normal human gastric epithelial cells, the expressions of PLEC, FAT1, EZR, HMGA1, MISP and PLAUR genes in gastric cancer cells were significantly upregulated( P <0.05). CCK8 experiment results showed that the cell reproductive capacity in the si-PLEC and si-PLAUR groups was significantly lower than that in the si-NC group(P<0.05). The tumor volume formed by gastric cancer cells with PLEC expression interference was significantly smaller than that of the control group, and the tumor growth rate was significantly slower. Conclusion: PLEC, FAT1, EZR, HMGA1,MISP and PLAUR play important roles in the occurrence and development of gastric cancer, and may serve as potential molecular biomarkers for early diagnosis and targeted treatment.
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基本信息:
DOI：10.19767/j.cnki.32-1412.2025.06.001
中图分类号:R735.2
引用信息:
[1]吴姜蓉,成翔宇,夏一鸣,等.胃癌细胞中细胞骨架结构相关基因的表达及其在胃癌发生发展中的作用[J].交通医学,2025,39(06):551-554.DOI:10.19767/j.cnki.32-1412.2025.06.001.
基金信息:
南通市科技计划项目（MS2023081）